Scientific Publications

February 8, 2017

A general [18F]AlF radiochemistry procedure on two automated synthesis platforms

L. Allott, C. Da Pieve, D. R. Turton and G. Smith

The aluminium fluoride-18 ([18F]AlF) radiolabelling procedure has generated great interest because it is a
simple, one-pot method that can be used to directly radiolabel small molecules, peptides and proteins
without the requirement for a [18F]fluoride drying step. Reported here is the development of an automated
[18F]AlF radiolabelling procedure of three different precursors (one small molecule and two peptides) on
two automated synthesis platforms: GE TRACERlab FXFN and Trasis AllInOne (AIO). Aiming at the clinical
translatability of a [18F]AlF radiosynthetic methodology, the use of both platforms yielded radioconjugates
with >98% radiochemical purity (RCP) within 26–35 min and required a single rapid purification step. The
Trasis AIO platform gave improved [18F]fluoride incorporation, and generally produced radioconjugates with a higher radiochemical yield (RCY) and effective specific activities (SA) when compared to the GE TRACERlab FXFN system.

DOI: 10.1039/c6re00204h

 

November 9, 2016

F-18 labelled PSMA-1007: biodistribution, radiation dosimetry and histopathological validation of tumor lesions in prostate cancer patients

Frederik L. Giesel & B. Hadaschik & J. Cardinale & J. Radtke & M. Vinsensia1 & W. Lehnert & C. Kesch & Y. Tolstov & S. Singer & N. Grabe & S. Duensing & M. Schäfer & O. C. Neels & W. Mier1 & U. Haberkorn1 & K. Kopka & C. Kratochwil

18F-PSMA-1007 produced on Trasis AllInOne automated synthesis module was proven to perform at least comparably to 68Ga-PSMA-11 and overcomes some practical limitations of 68Ga-labelled PSMA-targeted tracers.

DOI 10.1007/s00259-016-3573-4

October 10, 2016

Automation of the Radiosynthesis of Six Different 18F-labeled radiotracers on the AllinOne

Shihong Li, Alexander Schmitz, Hsiaoju Lee and Robert H. Mach*

The six radiopharmaceuticals were obtained in high yield and high specific activity with full automation on the AllinOne synthesis module under current good manufacturing practice (cGMP) guidelines. The study demonstrates the versatility of this synthesis module for the preparation of a wide variety of 18F-labeled radiopharmaceuticals for PET imaging studies.

DOI 10.1186/s41181-016-0018-0

August 30, 2016

Enabling Efficient PET Imaging of Synaptic Vesicle Glycoprotein 2A (SV2A) with a Robust and One-Step Radiosynthesis of a Highly Potent 18F‑Labeled Ligand ([18F]UCB-H)

Corentin Warnier,Christian Lemaire, Guillaume Becker, Guillermo Zaragoza, Fabrice Giacomelli, Joël Aerts, Muhammad Otabashi, Mohamed Ali Bahri, Joël Mercier, Alain Plenevaux, and André Luxen

The study describes the straightforward synthesis of a stable pyridyl(4-methoxyphenyl)iodonium salt and its [18F] radiolabeling on the Trasis AllinOne synthesizer within a one-step, fully automated and cGMP compliant radiosynthesis of [18F]UCB-H ([18F]7), a PET tracer for the imaging of synaptic vesicle glycoprotein 2A (SV2A).

DOI: 10.1021/acs.jmedchem.6b00905

August 16, 2016

[18F]MEL050 as a melanin-targeted PET tracer: Fully automated radiosynthesis and comparison to 18F-FDG for the detection of pigmented melanoma in mice primary subcutaneous tumors and pulmonary metastases

Nathalie Rizzo-Padoin, Michael Chaussard, Nicolas Vignal, Ewa Kotula, Vadim Tsoupko-Sitnikov, Sofia Vaz, Fortune Hontonnou, Wang-Qing Liu, Jean-Luc Poyet, Michel Vidal, Pascal Merlet, Benoit Hosten, Laure Sarda-Mantel

Successful implementation of the the radiosynthesis of [18F]MEL050 using the AllInOne module, including HPLC purification and formulation.

http://dx.doi.org/10.1016/j.nucmedbio.2016.08.010

July 20, 2016

Fully automated, robust and reproducible synthesis of 16α-18Ffluoroestradiol (18F-FES)

Guillaume BLUET, Cathy AUBERT, Florence GRUSS-LELEU, Laurent BESRET, Sébastien D’HEILLY, Sébastien ROY, Muhammad OTABASHI and Laurent BRICHARD

The study describes an in-house automated, reliable and reproducible radiosynthesis using the AllInOne synthesizer for the routine production of [18F]-FES for preclinical studies. The easy to use module allowed to develop efficient automated synthetic and purification procedures to obtain [18F]-FES in good radiochemical yields (up to 25% ndc) within just 45 min.

July 17, 2016

High-yield fully automated production of [18F]Fluoro-Choline at High Activity on the AllinOne Synthesizer

M, Otabashi; C, Desfours; T, Vergote; L, Brichard; JL, Morelle and G, Philippart - Trasis

A fully automated method was developed on the AllinOne synthesizer , with high and reproducible yields at all activity levels using ready-to-use kits. The results show no radioactive gases, extremely low levels of DMAE and compliance with any QC testing required. 

May 19, 2016

[ 68Ga] peptide high-output production on commercially available MiniAIO® synthesizer

Collet C., Remy S., Didier R., Vergote T., Véran N., Karcher G.

The conclusions of the study show: simple and reproductible synthesis methods without solvent, compatible with different peptide precursors, excellent radiochemical yield, each radiotracer was synthesized more than 10 times germanium concentration lower than the Ph.Eur. limit.

May 2, 2016

Development of [18F]Fluoro-C-glycosides to radiolabel peptides for PET application

Dr Charlotte Collet, Petry N., Chrétien F., Karcher G.,Pellegrini-Moïse N., Lamandé-LangleS - CHRU Nancy-Brabois

The radiochemistry research conducted by Dr. Collet Charlotte from the research group NancycloTep GIE (Université de Lorraine, CHRU Nancy, France), led by Professor KARCHER Gilles, has shown that very complex chemistry can be performed by using the whole set of capabilities and features of the AllinOne synthesizer. One process with two reactors, two HPLC, SPE prepurification and click chemistry on one automated synthesizer.